ABSTRACT
The purpose of this study was to clarify the strains of E .granulosus from sheep of Xiwuqi and people of Xil-inhot in Inner Mongolia region and its genotypes .CO1 and ND1 of mitochondria gene were cloned and sequenced ,and then they were analyzed by MegAlign of DNAStar5 .0 .Results showed that the length of CO1 and ND1 gene of E .granulosus ,which were from sheep of Xiwuqi or people of Xilinhot ,were 936 bp and 895 bp ,respectively .The homology of CO1 gene sequences of E .granulosus strains from Xiwuqi and Xinjiang was 99 .3% ,while the homology of the corresponding gene sequences of E .granulosus from man of Xilinhot City and Xinjiang were 98 .6% .ND1 gene of E .granulosus of sheep from Xiwuqi and hu-man from Xilinhot were identical to ND1 gene of G1 type .All these indicated that the homology of E .granulosus from the two regions were high and the genotype were G1 type ,which provided an important basis for the determination of strains ,and it al-so had a great significance to prevent and control the disease .
ABSTRACT
Objective Statistically analyze the serum P53 antibody level in lung cancer patients to show its diagnostic values.Methods 53 cases of lung cancer and 32 cases of lung cancer after operation and chemotherapy are used as study group. 34 normal controls and 35 cases of lung benign disease were used as control group. All the serum P53 antibody levels of the testees are measured with a ELISA kit.Results In 53 cases of lung cancer group, the positive rate was 58.5% (31/53), while the average serum P53 antibody level was (4.551?6.074)IU/ml. In 32 cases of lung cancer after treatment including surgical and medical procedures, the positive rate was 25% (8/32), while the average serum P53 antibody level was (1.086?2.836)IU/ml. In 35 cases lung benign disease group, the average serum P53 antibody level was (0.163?0.097)IU/ml. In normal group, the average serum P53 antibody level was (0.162?0.08)IU/mL.The lung cancer group had statistical significance in comparison with the other three groups (P0.05).Conclusion The P53 antibody′s appearance is highly correlated with lung cancer. It can be used as an important marker of lung cancer and be beneficial to the differential diagnosis of lung lesions and the evaluation of the treatment.